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Several months later, they gradually begin to produce sperm. During the first 18 to 24 months after birth, the testes do not produce sperm and the colt is infertile. Transmission Electron Microscopic Analysis of Sperm — This involves evaluating sperm morphology with a very high-powered microscope. Once the migration and maturation process is completed, the spermatozoa are stored in the tail of the epididymis. Conversely, some stallions exhibit decreased fertility even though the percentage of morphologically normal sperm in their ejaculates is high. It is here that spermatozoa complete the maturation process and are stored. Abnormally high semen pH can be associated with inflammatory lesions of the internal male genital tract.

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Once spermatozoa are released within the seminiferous tubule, they travel through a series of ducts to the epididymis, which is lightly attached to the upper surface of each testis.

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Karyotyping involves arranging computerized images, drawings, or photomicrographs of chromosomes. Thus, a strong case is made for ongoing semen analysis during a breeding season. It will remain at that weight and size during the first 10 months of life.

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